Thermo Fisher Scientific CD31 Monoclonal Antibody (ER-MP12)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50

-

ELISA (ELISA)

1:50

-

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

ER-MP12

Immunogen

Murine macrophage precursor hybrid W1C3cell hybrid

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1072120

Product Specific Information

MA1-40074 detects CD31 from mouse samples.
MA1-40074 reacts with an antigen present on cell subpopulations of adult mouse bone marrow, including pluripotent hematopoietic stem cells. MA1-40074 reacts with the major population of colony forming unit macrophage (CFU-M) precursor cells, with subpopulation of pre CFU-M and monoblasts, and with precursor cells of granulocytes.

MA1-40074 has been successfully used in ELISA, immunohistochemistry (paraffin and frozen), immunoprecipitation, and flow cytometry applications. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting, working dilution is 1:50.

The MA1-40074 immunogen is mouse PECAM-1/CD31.

Suggested positive control: Macrophage cell line RMB-1.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.