Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Biotin, eBioscience
Mouse CD62L (L-Selectin) 단백질을 인식하는 MEL-14 클론의 Biotin 결합 단일클론 항체. Flow cytometry 및 면역세포 분석에 적합. 고순도 Affinity chromatography 정제, 0.5 mg/mL 농도. 연구용으로만 사용.
- 카탈로그번호
- 13-0621-8x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Biotin, eBioscience
Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (MEL-14), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 55 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEL-14 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466491 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
- Alexa Fluor 561
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV series (395–805)
- Brilliant Violet series (421–786)
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue/Red/Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright series (436–780)
- Request custom conjugation
Product Specific Information
The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, sulfated sialylated glycoproteins such as CD34, GlyCAM-1, and MAdCAM-1, mediating lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.
Applications Reported:
The MEL-14 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.25 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.
Target Information
Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein adhesion receptor composed of:
- Aminoterminal C-type lectin binding domain
- EGF-like domain
- Two short consensus repeat (SCR) sequences
- Spacer region
- Transmembrane region
- Short cytoplasmic region
It is constitutively expressed on leukocytes including lymphocytes (except some memory T cells), monocytes, and polymorphonuclear cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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