Thermo Fisher Scientific GFAP (Astrocyte and Neural Stem Cell Marker) Monoclonal Antibody (GFAP/6879)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Stability: Antibody is stable for 24 months
• Positive Control: Human brain or astrocytoma
• Cellular Localization: Cytoplasm

Specificity Comments:
This monoclonal antibody recognizes a protein of approximately 50 kDa, identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia and is a well-established marker for benign astrocyte and neoplastic cells of glial origin in the central nervous system.
Antibody to GFAP is useful for differentiating primary gliomas from metastatic brain lesions and for documenting astrocytic differentiation in tumors outside the CNS.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. It is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, satellite cells of peripheral ganglia, and non-myelinating Schwann cells of peripheral nerves. Neural stem cells also strongly express GFAP.

Antibodies to GFAP are widely used as markers of astrocytic cells. Many types of brain tumors derived from astrocytic cells express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, salivary tumor cells, and sometimes in erythrocytes. GFAP is used to distinguish astrocytes from other glial cells during development.
Mutations in the GFAP gene cause Alexander disease, a rare disorder of astrocytes in the CNS. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.