Thermo Fisher Scientific IL4R (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-4 Receptor alpha. Anti-Human sIL-4 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human sIL-4 Receptor alpha by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sIL-4 Receptor alpha (500-P325) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human sIL-4 Receptor alpha.

Western Blot:
To detect Human sIL-4 Receptor alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human sIL-4 Receptor alpha is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P325BT-1MG will be provided as 2 × 500 µg.

Target Information

IL4 Receptor alpha (IL4RA):
The IL4RA is the alpha chain of the interleukin 4 receptor, which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine, and mucus production at sites of allergic inflammation. The IL4 response promotes Th2 differentiation. The secreted extracellular domain of IL4R alpha (sIL4R alpha) can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells, but has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, including allergic rhinitis, sinusitis, asthma, and eczema.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.