Thermo Fisher Scientific IL4R (soluble) Polyclonal Antibody, Biotin, PeproTech
Human IL-4 Receptor alpha(sIL4R) 인식용 Biotin 표지 토끼 다클론 항체로, Western Blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 재조합 단백질로 면역화되었습니다. 알레르기 염증 관련 연구에 유용합니다.
- 카탈로그번호
- 500-P325BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL4R (soluble) Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO cells-derived Recombinant Human IL-4 Receptor alpha |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930426 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-4 Receptor alpha. Anti-Human sIL-4 Receptor alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human sIL-4 Receptor alpha by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sIL-4 Receptor alpha (500-P325) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human sIL-4 Receptor alpha.
Western Blot:
To detect Human sIL-4 Receptor alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human sIL-4 Receptor alpha is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Packaging:
500-P325BT-1MG will be provided as 2 × 500 µg.
Target Information
IL4 Receptor alpha (IL4RA):
The IL4RA is the alpha chain of the interleukin 4 receptor, which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine, and mucus production at sites of allergic inflammation. The IL4 response promotes Th2 differentiation. The secreted extracellular domain of IL4R alpha (sIL4R alpha) can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells, but has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, including allergic rhinitis, sinusitis, asthma, and eczema.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.