Thermo Fisher Scientific MMP3 Polyclonal Antibody, PeproTech
Rabbit polyclonal antibody specific for human MMP3, validated for ELISA and Western blot. Affinity-purified for high specificity and sensitivity. Lyophilized form, stored at -20°C, suitable for research use only.
- 카탈로그번호
- 500-P324-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MMP3 Polyclonal Antibody, PeproTech
Applications
Western Blot (WB)
- Tested Dilution: 0.1–0.2 µg/mL
ELISA
- Tested Dilution: 0.5–2.0 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human MMP-3 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930415 |
Product Specific Information
AA Sequence of recombinant protein:
MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MMP-3. Anti-Human MMP-3-specific antibody was purified by affinity chromatography employing an immobilized Human MMP-3 matrix.
Sandwich ELISA:
To detect Human MMP-3 by sandwich ELISA (using 100 µL/well), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MMP-3 (500-P324BT) as a detection antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human MMP-3.
Western Blot:
To detect Human MMP-3 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human MMP-3 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
MMP3 (stromelysin-1) belongs to the peptidase M10A subfamily. It is secreted in its pro-protein form and activated by extracellular proteinase cleavage. MMP3 binds zinc and calcium ions and has metallo-endopeptidase activity. The active enzyme degrades collagen, gelatin, fibronectin, and laminin, and is involved in wound repair and tissue remodeling. Mutations in the gene can result in coronary heart disease 6.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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