Thermo Fisher Scientific Human TLR-4 Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

ESWEPCVEVV PNITYQCMEL NFYKIPDNLP FSTKNLDLSF NPLRHLGSYS FFSFPELQVL DLSRCEIQTI EDGAYQSLSH LSTLILTGNP IQSLALGAFS GLSSLQKLVA VETNLASLEN FPIGHLKTLK ELNVAHNLIQ SFKLPEYFSN LTNLEHLDLS SNKIQSIYCT DLRVLHQMPL LNLSLDLSLN PMNFIQPGAF KEIRLHKLTL RNNFDSLNVM KTCIQGLAGL EVHRLVLGEF RNEGNLEKFD KSALEGLCNL TIEEFRLAYL DYYLDDIIDL FNCLTNVSSF SLVSVTIERV KDFSYNFGWQ HLELVNCKFG QFPTLKLKSL KRLTFTSNKG GNAFSEVDLP SLEFLDLSRN GLSFKGCCSQ SDFGTTSLKY LDLSFNGVIT MSSNFLGLEQ LEHLDFQHSN LKQMSEFSVF LSLRNLIYLD ISHTHTRVAF NGIFNGLSSL EVLKMAGNSF QENFLPDIFT ELRNLTFLDL SQCQLEQLSP TAFNSLSSLQ VLNMSHNNFF SLDTFPYKCL NSLQVLDYSL NHIMTSKKQE LQHFPSSLAF LNLTQNDFAC TCEHQSFLQW IKDQRQLLVE VERMECATPS DKQGMPVLSL NITCQMNKT

Molecular weight

69.3 kDa

Class

Recombinant

Type

Protein

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to bind rhMD-2 in a functional ELISA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human TLR-4 is a 69.3 kDa glycoprotein containing 609 amino acid residues of the TLR-4 extracellular domain. As a result of glycosylation, Recombinant Human TLR-4 migrates with an apparent molecular mass of approximately 90-100 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.