Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Functional Grade, eBioscience
인간 CD3e를 인식하는 UCHT1 클론 단일클론 항체로 T세포 활성화 및 유세포분석에 적합. Functional Grade로 제조되어 세포 활성화 실험에 사용 가능. 높은 순도와 낮은 엔도톡신 수준, 무보존제 PBS 용액 형태로 제공.
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- 16-0038-xxx (5개 옵션)
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5개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Functional Grade, eBioscience
Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 34 publications |
| Functional Assay | Assay-Dependent | View 10 publications |
| T-Cell Activation (TCA) | - | View 2 publications |
| In vitro Assay (IV) | - | View 2 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT1 |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865588 |
Available Conjugates
- Unconjugated
- Alexa Fluor 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395~805
- Brilliant Violet 421~786
- eFluor 450, 506
- FITC
- NovaFluor Blue, Red, UV, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- Super Bright 436~780
- Request custom conjugation
Product Specific Information
The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with CD3 gamma and delta, is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling leading to activation and proliferation.
- Applications Reported: Flow cytometric analysis and T cell activation.
- Applications Tested: Tested by flow cytometry using normal human peripheral blood cells (≤1 µg/test).
- A test is defined as the amount of antibody staining a sample in 100 µL volume.
- Recommended to titrate for optimal performance.
- Storage and Handling: Use in sterile environment.
- Filtration: 0.2 µm post-manufacturing filtered.
- Purity: >90% (SDS-PAGE).
- Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
- Aggregation: <10% (HPLC).
Target Information
The CD3 complex is crucial for transducing antigen-recognition signals into T cells and regulating surface expression of the TCR complex. It comprises CD3 gamma, delta, epsilon, and zeta subunits, encoded by genes on human chromosome 11. These subunits contain cytoplasmic tails with tyrosine-based motifs mediating signal transduction. CD3 is expressed on most peripheral blood lymphocytes and thymocytes, but not on B or NK cells. Defects in CD3 genes are linked to CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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