Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Functional Grade, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugates

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395~805
• Brilliant Violet 421~786
• eFluor 450, 506
• FITC
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright 436~780
• Request custom conjugation

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with CD3 gamma and delta, is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling leading to activation and proliferation.

• Applications Reported: Flow cytometric analysis and T cell activation.
• Applications Tested: Tested by flow cytometry using normal human peripheral blood cells (≤1 µg/test).
  • A test is defined as the amount of antibody staining a sample in 100 µL volume.
  • Recommended to titrate for optimal performance.
• Storage and Handling: Use in sterile environment.
• Filtration: 0.2 µm post-manufacturing filtered.
• Purity: >90% (SDS-PAGE).
• Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
• Aggregation: <10% (HPLC).

Target Information

The CD3 complex is crucial for transducing antigen-recognition signals into T cells and regulating surface expression of the TCR complex. It comprises CD3 gamma, delta, epsilon, and zeta subunits, encoded by genes on human chromosome 11. These subunits contain cytoplasmic tails with tyrosine-based motifs mediating signal transduction. CD3 is expressed on most peripheral blood lymphocytes and thymocytes, but not on B or NK cells. Defects in CD3 genes are linked to CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.