Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 488, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV Series (395, 563, 615, 661, 737, 805)
• Brilliant Violet Series (421, 605)
• eFluor 450, 506, 660
• FITC
• NovaFluor Blue (510, 585), NovaFluor Yellow (590, 660, 730)
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright Series (436, 600, 645, 702, 780)
• Custom conjugation available upon request

Product Specific Information

The OKT3 monoclonal antibody recognizes an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties in vivo and has been effective in treating renal, heart, and liver allograft rejection.

CD3 subunits (gamma, delta, epsilon) are essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates intracellular signaling leading to cellular activation and proliferation.

Applications Reported:

• Flow cytometric analysis
• In vitro activation of T cells

Applications Tested:

• Tested by flow cytometric analysis of normal human peripheral blood cells
• ≤0.25 µg/test (100 µL volume, 10⁵–10⁸ cells/test)
• Optimal titration recommended for specific assays

Storage and Handling:

• Use in sterile conditions
• 0.2 µm post-manufacturing filtered

Purity and Quality:

• Purity: >90% (SDS-PAGE)
• Endotoxin: <0.001 ng/µg (LAL assay)
• Aggregation: <10% (HPLC)

Target Information

The CD3 complex is crucial for transducing antigen-recognition signals into the cytoplasm of T cells and regulating TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, which are immunoglobulin superfamily members encoded on human chromosome 11.

CD3 subunits associate with signal transduction molecules via a double tyrosine-based motif. CD3 plays roles in TCR-induced growth arrest, survival, and proliferation. CD3 antigen is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells.

Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.