Thermo Fisher Scientific NIT1 Monoclonal Antibody (OTI4D12), TrueMAB
인간 NIT1 단백질을 표적으로 하는 단일클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 적합합니다. 고순도 친화 크로마토그래피로 정제된 무보존제 제품이며, 세포 성장 및 아폽토시스 연구에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D12 |
| Immunogen | Full length human recombinant protein of human NIT1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
NIT1 plays a role in cell growth and apoptosis; loss of expression promotes cell growth and resistance to DNA damage stress.
It has tumor suppressor properties that enhance apoptotic responsiveness in cancer cells, acting additively with FHIT.
NIT1 negatively regulates primary T-cells and lacks omega-amidase activity like NIT2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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