Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Recombinant Rabbit Monoclonal Antibody (HisH2AXS139-1B3)

Applications

Product Specifications

Additional Formats

• APC
• PE
• Request custom conjugation

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire

Target Information

Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome particle. In eukaryotes, DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the carboxy terminus of H2AX, resulting in γ-H2AX.
Phosphorylation can be detected by Western blot or immunofluorescence, indicating DSB frequency.
Phosphatidylinositol 3-kinases are implicated in H2AX phosphorylation, though ATM, DNA-PK, and ATR may also contribute.
H2A.X contains 143 amino acids, synthesized in G1 and S-phase. Its mRNA exists in two forms (polyA+ and polyA−), corresponding to basal and replication-type histone mRNAs.
Histone H2A.X maps to human chromosome region 11q23.2–q23.3 and contributes to DNA structure and repair, regulating MDC1 interaction at DNA damage sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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