Thermo Fisher Scientific Phospho-Histone H3 (Ser28) Recombinant Rabbit Monoclonal Antibody (HisH3S28-D6), APC
APC 형광 표지된 Phospho-Histone H3 (Ser28) 재조합 토끼 단클론 항체로 세포 분석(FACS)에 최적화. 인간 및 마우스 반응성, 높은 특이성과 일관성 제공. Protein A/G 정제, PBS 완충액에 BSA 및 sodium azide 포함. 연구용으로만 사용.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HisH3S28-D6 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745018 |
Additional Formats
- Unconjugated (MA5-36939)
- FITC (MA5-36941)
- PE (MA5-36940)
- Request custom conjugation (Custom Form)
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. It, along with other core histones, forms nucleosomes that structure chromatin.
Histones are central to transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications such as methylation, acetylation, and phosphorylation influence chromatin structure and transcription factor binding.
The N-terminal tail of histone H3 undergoes these modifications, affecting various cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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