Thermo Fisher Scientific VDR Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Predicted to react with dog, mouse, and rat based on sequence homology.
• In Western blot, this antibody detects a band at ~40–45 kDa in human brain cell lysate.
• A minimum incubation time of 1 hour is recommended.
• Goat anti-Human Vitamin D Receptor antibody recognizes human vitamin D nuclear hormone receptor (VDR), an approximately 48 kDa member of the trans-acting transcriptional regulatory factors.

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to DNA sequences following activation by appropriate hormones.
The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors, including estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors.

Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation.
VDR is found in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system.

Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression occurs when VDR binds within the IL-2 enhancer.
Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)₂D₃ interferes with assembly of NFATp/Fos/Jun/DNA complex and subsequent IL-2 gene transcription.
The corresponding gene for the vitamin D receptor is NR1I1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.