Thermo Fisher Scientific Osteopontin Polyclonal Antibody
인간 Osteopontin 단백질을 인식하는 염소 유래 polyclonal 항체로, Western blot 및 ELISA에 적합합니다. C-말단 펩타이드를 면역원으로 사용하였으며, 친화 크로마토그래피로 정제되었습니다. TBS 버퍼에 0.5% BSA 및 0.02% sodium azide를 포함하며, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| ELISA | 1:64000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide with sequence C-DPKSKEEDKHLK, from the C-terminal of osteopontin (aa 288–299) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2239842 |
Product Specific Information
- Predicted to react with dog, mouse, and rat based on sequence homology.
- Western blot detects a band of approximately 60 kDa in lysates of human thymus, tonsil, and spleen.
- Goat anti-Human Osteopontin antibody recognizes an epitope within the C-terminal (CT) region of osteopontin, an acid-rich, multidomain, phosphorylated glycoprotein (~60 kDa).
Target Information
Osteopontin is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. It is a multifunctional protein involved in bone mineralization, cell adhesion, cell migration, chronic inflammatory disease, and transformation. Proteolytic cleavage by thrombin and matrix metalloproteinases near the integrin-binding Arg-Gly-Asp sequence modulates the function of OPN and its integrin-binding properties. Thrombin-cleaved fragments of Osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage and serve as a novel substrate for plasmin and cathepsin D.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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