Thermo Fisher Scientific alpha Tubulin Polyclonal Antibody
Rabbit polyclonal antibody targeting human alpha Tubulin for WB, IHC, and ICC applications. High specificity and broad predicted reactivity across multiple species. Supplied as liquid, purified by antigen affinity chromatography, and ideal for cytoskel...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Tubulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.43 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792437 |
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (99%), Xenopus laevis (98%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
Positive Control: 293T, A431, HeLa, HepG2.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Alpha Tubulin is part of a heterodimer consisting of alpha and beta tubulin subunits. These tubulins (~55 kDa each) form the basic building blocks of microtubules—cylindrical filamentous structures found in nearly all eukaryotic cells. Microtubules provide structural support, facilitate intracellular transport, and play a key role in mitosis.
Alpha and beta tubulins are highly conserved across species, and the alpha tubulin gene is similar to the mouse and rat Tuba1 gene. Expression is predominantly found in morphologically differentiated neurologic cells.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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