Thermo Fisher Scientific Phospho-Paxillin (Tyr118) Monoclonal Antibody (GT712)
Phospho-Paxillin (Tyr118) 단일클론 항체로 인간, 생쥐, 랫트 시료에서 사용 가능. WB, IHC, ICC/IF 등 다양한 응용에 적합. 단백질 G 정제, PBS 버퍼, 보존제 없음. 세포 부착 및 신호전달 연구용으로 최적화된 연구용 시약.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500–1:3,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT712 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Paxillin (phospho Tyr118). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2735317 |
Product Specific Information
- Positive Control: Rat2, A431 (1 mM Pervanadate treatment for 0.5 hr)
- Predicted Reactivity: Mouse (100%), Chicken (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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