Thermo Fisher Scientific CYP3A4 Polyclonal Antibody
CYP3A4 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA 등에 사용 가능. 인간, 마우스, 랫트 반응성. 액상 형태로 -20°C 보관. 약물 대사 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 244–503 of human CYP3A4 (NP_059488.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.12 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914392 |
Product Specific Information
- Positive test controls: Mouse liver, Rat liver
- Localization: Endoplasmic reticulum membrane, Microsome membrane, Single-pass membrane protein
Immunogen sequence:
EVTNFLRKSV KRMKESRLED TQKHRVDFLQ LMIDSQNSKE TESHKALSDL ELVAQSIIFI FAGYETTSSV LSFIMYELAT HPDVQQKLQE EIDAVLPNKA PPTYDTVLQM EYLDMVVNET LRLFPIAMRL ERVCKKDVEI NGMFIPKGVV VMIPSYALHR DPKYWTEPEK FLPERFSKKN KDNIDPYIYT PFGSGPRNCI GMRFALMNMK LALIRVLQNF SFKPCKETQI PLKLSLGGLL QPEKPVVLKV ESRDGTVSGA
Target Information
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids.
This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and certain pharmacological agents.
CYP3A4 enzyme is involved in the metabolism of approximately half of the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam, and erythromycin. It also metabolizes steroids and carcinogens.
This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously, CYP3A3 was thought to exist, but is now considered a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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