Thermo Fisher Scientific CD284 (TLR4) Monoclonal Antibody (HTA125), Biotin, eBioscience

Thermo Fisher Scientific CD284 (TLR4) Monoclonal Antibody (HTA125), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). TLR family members are type I transmembrane proteins involved in innate immunity and inflammation. TLR4 associates with MD-2 and CD14 to mediate LPS recognition and signaling. It is expressed on peripheral blood monocytes and can immunoprecipitate human TLR4 (~100 kDa) from transfected cells.

For optimal staining of TLR4, use the lysed whole blood protocol rather than Ficoll-gradient prepared peripheral blood cells, as gradient use reduces staining intensity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of normal human peripheral blood cells (≤1 µg/test, 100 µL final volume, 10⁵–10⁸ cells/test).
  Careful titration is recommended for optimal performance.

Target Information

TLR4 is a member of the Toll-like receptor family involved in pathogen recognition and activation of innate immunity. It is expressed in placenta and myelomonocytic leukocytes, forming part of the LPS receptor complex with CD14 and LY96. Mutations in TLR4 affect LPS responsiveness. TLR4-deficient mice show increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.