Thermo Fisher Scientific CD282 (TLR2) Monoclonal Antibody (TL2.1), Biotin, eBioscience

Thermo Fisher Scientific CD282 (TLR2) Monoclonal Antibody (TL2.1), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). TLR2 is a type I transmembrane protein involved in innate immunity and inflammation, expressed by peripheral blood monocytes. TL2.1 is a blocking antibody used to study TLR2’s role in microbial lipoprotein/lipopeptide-induced cytokine production. It immunoprecipitates human TLR2 (~90 kDa) from PBMC and HMEC.

Applications Reported: TL2.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤1 µg/test in 100 µL final volume. Optimal performance should be determined empirically.

Target Information

TLR2 is part of the Toll-like receptor family, crucial for pathogen recognition and activation of innate immunity. It forms heterodimers with TLR1 or TLR6 to recognize bacterial and mycoplasma lipoproteins. TLR2 is highly expressed in peripheral blood leukocytes and mediates host response to Gram-positive bacteria and yeast via NF-kappaB activation.

Expression is highest in CD14+ monocytes, followed by granulocytes, B-cells, and T-cells. Cytokines such as LPS, GM-CSF, IL-1, and IL-10 upregulate TLR2, while IL-4, IFN-gamma, and TNF downregulate its expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.