Thermo Fisher Scientific 53BP1 Polyclonal Antibody, DyLight 550
다양한 응용(WB, IHC, ICC, Flow)에 적합한 53BP1 폴리클로날 항체입니다. DyLight 550 형광 표지로 우수한 신호 감지 가능. 인간 및 생쥐 시료 반응. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 2–5 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a portion of human 53BP1 encoded in exon 11, 12 and 19 [Uniprot: Q12888] (aa 12–19). |
| Conjugate | DyLight™ 550 |
| Excitation/Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.61 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153558 |
Product Specific Information
This antibody reacts with Human samples in Western blot and ICC applications, and with Mouse in ICC applications.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to double-strand break sites. It may play a role in checkpoint signaling during mitosis, enhance TP53-mediated transcriptional activation, and participate in DNA repair to maintain genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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