Thermo Fisher Scientific FAS (soluble) Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific FAS (soluble) Polyclonal Antibody, Biotin

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sFas Receptor. Anti-Human sFas Receptor-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human sFas Receptor by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sFas Receptor (500-P295) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human sFas Receptor.

Western Blot:
To detect Human sFas Receptor by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 mg/mL. When used with compatible development reagents, the detection limit for Recombinant Human sFas Receptor is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P295BT-1MG will be provided as 2 × 500 µg.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein functioning as a cell death receptor of the TNFR superfamily. It also exists in a soluble form (~26 kDa). Stimulation of FAS results in aggregation of FADD domains, formation of the death-inducing signaling complex (DISC), and activation of caspases, leading to apoptosis.
FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in proliferative signaling in fibroblasts and T cells. Multiple splice variants exist, some lacking the transmembrane domain and negatively regulating apoptosis.
The Fas/Fas ligand system is implicated in diseases such as AIDS, hepatitis, and cancer, and plays a role in antiviral immune responses.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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