Thermo Fisher Scientific IL-9 Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL
• Publications: 1 publication available

In vitro Assay (IV)

• Tested Dilution: Not specified
• Publications: 1 publication available

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MQGCPTLAGI LDINFLINKM QEDPASKCHC SANVTSCLCL GIPSDNCTRP CFSERLSQMT NTTMQTRYPL IFSRVKKSVE VLKNNKCPYF SCEQPCNQTT AGNALTFLKS LLEIFQKEKM RGMRGKI

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-9. Anti-Human IL-9-specific antibody was purified by affinity chromatography employing an immobilized Human IL-9 matrix.

Sandwich ELISA:
To detect Human IL-9 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-9 (500-P29Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human IL-9.

Western Blot:
To detect Human IL-9 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IL-9 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells.
The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated.
Recently, TGF-β has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

제품 이미지

• (500-P29-100UG_IL-9_P15248-1_Rabbit.svg)
• (500-P29-100UG_IL-9_P15248-1_Rabbit_PDP.jpeg)