Thermo Fisher Scientific EBP50 Recombinant Rabbit Monoclonal Antibody (JJ0946)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages include:

• Superior specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the rabbit immune repertoire

Target Information

NHERF1 (Na⁺/H⁺ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein associated with β-catenin and required for its localization at cell-cell junctions. It interacts with G protein-coupled receptors and regulates their trafficking, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport.
NHERF1/EBP50 inhibits cell motility and suppresses anchorage-independent growth. It contains a C-terminal ERM-binding region and two N-terminal PDZ domains, allowing head-to-tail intramolecular conformation for regulation of interactions.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.