Thermo Fisher Scientific Phospho-RPH3A (Ser424) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Rabphilin 3A only when phosphorylated at Ser424.

Target Information

The carboxy-terminal of Rabphilin-3A consists of two C2 domains (A and B), and the amino-terminal region (residues 45–170) contains a cysteine-rich region with two zinc finger motifs. Rabphilin-3A belongs to the C-type tandem C2 protein family, which includes synaptotagmins and Doc2.

Rabphilin-3A is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. It binds Rab3a/GTP/Mg²⁺ within amino-terminal residues 45–170 and interacts with calcium ions and phosphatidylinositol 4,5-bisphosphate-containing lipid vesicles via its C2 domains.

Rabphilin-3A acts as a positive regulator of calcium-dependent exocytosis, while Rab3a negatively regulates exocytosis. Although Rabphilin-3A associates with Rab3a, both influence exocytosis independently. Rabphilin-3A effects are likely mediated through interactions with an unknown factor recognizing the Rab3 binding domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.