Thermo Fisher Scientific Armenian Hamster IgG Isotype Control (eBio299Arm), PE-eFluor 610, eBioscience
Armenian hamster IgG 동형 대조 항체로, Flow Cytometry용으로 최적화된 PE-eFluor 610 결합 단일클론 항체입니다. 실험 항체와 동일 농도로 사용하여 비특이적 신호를 구분하며, 4°C 암소 보관 및 광산화에 주의가 필요합니다.
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Applications
Tested Dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBio299Arm |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2637313 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 615 / 660
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
Description:
The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Use the isotype control at the same concentration as the experimental antibody.
Fluorochrome Information:
PE-eFluor® 610 can be excited with laser lines from 488–561 nm and emits at 607 nm. Recommended filter: 610/20 band pass (PE-Texas Red® equivalent). Ensure instrument compatibility.
Light Sensitivity:
Sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.
Excitation/Emission/Laser:
488–561 nm / 607 nm; Blue, Green, Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Isotype control antibodies serve as negative controls to distinguish non-specific background signal from specific antibody signal.
They are useful in:
- Flow Cytometry
- Chromatin Immunoprecipitation (ChIP)
- Immunohistochemistry
- Gel shift assays
Isotype controls should match the species and isotype of the primary antibody and, if directly labeled, use the same conjugate for accurate comparison.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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