Thermo Fisher Scientific CDK2 Monoclonal Antibody (OTI1C4), TrueMAB
Human CDK2 단백질을 인식하는 Mouse IgG2a 단일클론 항체로, Western blot, ICC/IF, Flow cytometry에 사용 가능. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도 유지. 연구용으로 세포주기 관련 단백질 분석에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C4 |
| Immunogen | Full-length human recombinant CDK2 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CDK2/cyclinO is a member of the cyclin-dependent protein kinase family complexed with CyclinO. CyclinO, a protein isoform of the UNG gene, is localized in the nucleus and exhibits uracil-DNA glycosylase activity. CDK2/cyclinO levels increase during the G1 phase and are regulated throughout the cell cycle. Cyclin A–CDK2 levels peak at the G1/S transition, where both are associated with DNA in the initiation complex during replication. Rb protein acts as a substrate for CDK2–Cyclin E and/or CDK2–Cyclin A in vivo. CDK2 is activated and localized to the nucleus during late G1, S, and G2 phases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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