Thermo Fisher Scientific p53 Monoclonal Antibody (OTI1F4), TrueMAB

Thermo Fisher Scientific p53 Monoclonal Antibody (OTI1F4), TrueMAB

Applications and Tested Dilutions

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 activation leads to transcription of genes such as p21waf1, contributing to G1 or G2/M arrest.

Structurally, p53 includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization, while phosphorylation at Ser392 is associated with tumor formation. p53 functions as a key anticancer agent by inhibiting proliferation of damaged cells. It is also linked to aging, oxidative stress, and learning/memory deficits.
Mutations in TP53 disrupt DNA binding and tumor suppressor activity, contributing to malignancy. Germline mutations are associated with cancer-prone syndromes such as Li-Fraumeni.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.