Thermo Fisher Scientific DDIT3 Monoclonal Antibody (OTI4C4), TrueMAB
Human DDIT3 단백질을 인식하는 Mouse monoclonal antibody (OTI4C4). Western blot에 검증됨. 동결건조 형태로 제공되며, PBS 기반 버퍼에 8% trehalose 포함. 세포 스트레스 반응 연구에 적합.
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Applications
- Western Blot (WB): 1:2,000 dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C4 |
| Immunogen | Full length human recombinant protein of human DDIT3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
GADD153 (also known as DDIT3) is a small nuclear protein that dimerizes with transcription factors C/EBP alpha and beta.
This complex inhibits the normal binding of C/EBP to classical binding sites, while gaining activity toward non-classical, stress-related targets.
Under normal conditions, GADD153 expression is minimal, but it is highly upregulated under cellular or ER stress (e.g., tunicamycin treatment, nutrient starvation, or reducing agents affecting ER calcium flux).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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