Thermo Fisher Scientific CD4 Monoclonal Antibody (OTI1G10), TrueMAB
인간 CD4 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot과 IHC(P)에서 검증됨. 비결합(Unconjugated) 상태로 제공되며, 동결건조 형태로 보관. PBS 완충액(8% 트레할로스) 포함, -20°C에서 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G10 |
| Immunogen | Full length human recombinant protein of human CD4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The CD4 antigen participates in the recognition of MHC class II molecules and serves as a co-receptor for HIV. CD4 is primarily expressed in T helper cells, but can also be found in monocytes, macrophages, and dendritic cells.
At the tissue level, CD4 expression is detected in thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut.
It plays a key role in T-cell activation via association with the T-cell receptor complex and the protein tyrosine kinase Lck.
CD4 may also mediate neuronal damage in infectious and immune-mediated CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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