Thermo Fisher Scientific TNFRSF8 Monoclonal Antibody (OTI1G2), TrueMAB

Thermo Fisher Scientific TNFRSF8 Monoclonal Antibody (OTI1G2), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

CD30 (Ki-1, TNF Receptor Superfamily Member 8) is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily.
Originally identified as a surface antigen of Hodgkin’s and Reed–Sternberg cells, CD30 binds to its ligand CD30L (CD153) to mediate various cellular processes including proliferation, activation, differentiation, and apoptosis.

CD30 plays a critical role in the pathophysiology of Hodgkin’s disease and other CD30+ lymphomas, and acts as a costimulatory molecule in thymic negative selection. It is expressed on Hodgkin’s and Reed–Sternberg cells, certain non-Hodgkin’s lymphomas (e.g., Burkitt’s lymphoma), virus-infected T/B cells, and activated normal T/B cells.

In T cells, CD30 is found in subsets producing Th2-type cytokines and on CD4+/CD8+ thymocytes co-expressing CD45RO and the IL4 receptor. The soluble form (sCD30) serves as a marker of Th2 immune response.
TRAF2 and TRAF5 interact with CD30 to activate NF-κB signaling, promoting apoptosis and limiting autoreactive CD8 effector T-cell proliferation.
Two alternatively spliced transcript variants encoding distinct isoforms have been identified.

Expression: Mononuclear cells in Hodgkin’s lymphoma, Reed–Sternberg cells, and most Anaplastic Large Cell Lymphomas (ALCL); also expressed by embryonal carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.