Thermo Fisher Scientific DDIT3 Monoclonal Antibody (OTI4G2), TrueMAB
Western blot에 최적화된 인간 DDIT3 단클론 항체로, Mouse IgG1 형식의 TrueMAB 제품입니다. E. coli에서 발현된 full-length human DDIT3 항원을 면역원으로 사용하였으며, 동결건조 형태로 제공되어 재구성 후 실험에 바로 사용 가능합니다. 연구용 전용 시약입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4G2 |
| Immunogen | Full length human recombinant protein of human DDIT3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
GADD153 (also known as DDIT3) is a small nuclear protein that can dimerize with transcription factors C/EBP alpha and beta. The resulting complex inhibits normal C/EBP binding and function, while gaining binding activity to non-classical C/EBP stress-related targets. Under normal conditions, GADD153 expression is low but is highly upregulated during cellular or ER stress, such as treatment with tunicamycin, nutrient starvation, or reducing agents that disrupt calcium flux across the ER membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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