Thermo Fisher Scientific HDEL Monoclonal Antibody (2E7), PerCP
HDEL 모노클로날 항체(2E7), PerCP 결합형으로 효모, 식물, 효모 단백질의 ER 유지 신호(HDEL) 검출에 적합. Western blot 및 면역세포염색에 사용 가능. 1 mg/mL 농도, Protein G 정제, 4°C 보관. 연구용 한정.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Plant, Yeast |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E7 |
| Immunogen | Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931696 |
Available Formats
Product Specific Information
1 µg/mL of MA5-45242 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 78 kDa.
Target Information
The HSP70 family comprises four highly conserved proteins—HSP70, HSC70, GRP75, and GRP78—which serve as molecular chaperones. They facilitate multi-protein complex assembly, assist in protein translocation across membranes, and aid in proper folding of nascent polypeptides.
GRP78 is localized in the endoplasmic reticulum (ER), where it assists in folding and translocation of secretory proteins. Sorting of these proteins depends on a C-terminal tetrapeptide signal—KDEL in animal cells and HDEL in S. cerevisiae.
The 2E7 clone recognizes the C-terminal peptide HDEL, a common ER retention signal found in yeast, plants, nematodes, and other ER proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum, and wheat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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