Thermo Fisher Scientific ATG5 Polyclonal Antibody, Biotin
ATG5 단백질을 인식하는 Biotin 결합 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 연구 응용에 적합. 인간 ATG5 N-말단 펩타이드 기반 면역원 사용. 4°C 암소 보관, 0.8 mg/mL 농도.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to N-terminal region (within residues 1–50) of human ATG5 protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153748 |
Product Specific Information
- In Western blot, a band is observed around 56 kDa representing the ATG5-ATG12 complex (human ATG5 ≈ 33 kDa).
- Antigen retrieval with sodium citrate buffer (pH 6.0) is recommended prior to immunostaining paraffin tissues.
- Suggested positive control: mouse wildtype ES cell lysate.
Target Information
ATG5 (Autophagy Related 5) plays a critical role in autophagy and apoptotic processes. It is involved in:
- Mitochondrial quality control after oxidative damage
- Negative regulation of innate antiviral immune response
- Lymphocyte development and proliferation
- MHC II antigen presentation and adipocyte differentiation
Upon conjugation with ATG12, the complex contributes to autophagosome formation. ATG5 also interacts with FADD, influencing autophagic cell death. The ATG5-ATG12 conjugate associates with immune response proteins such as RIG-I and VISA, modulating type I interferon production and viral replication. Dysfunction of ATG5 is linked to diseases such as spinocerebellar ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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