Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), eBioscience

Applications

Product Specifications

Available Conjugated Formats

• Biotin
• Brilliant UV 805
• eFluor 450
• eFluor 660
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation available

Product Specific Information

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low-affinity receptor for IgE and plays a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.

Applications Reported:
The B3B4 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining.

Applications Tested:
The B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes. It can be used at ≤0.5 µg per test (defined as the amount of antibody staining a 100 µL cell sample). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD23 is a 45 kDa glycoprotein present on a subpopulation of peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. It is identical to the low-affinity IgE receptor found on B cells. CD23 expression has been detected in neoplastic cells from B cell chronic lymphocytic leukemia and certain lymphomas. Functionally, CD23 regulates B cell growth, differentiation, and IgE production. The soluble form of CD23 acts as a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.