Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), eBioscience
마우스 CD24 단백질을 인식하는 M1/69 클론 단일클론 항체로, Flow Cytometry, Western Blot, IHC 등 다양한 응용에 적합합니다. 비결합형 형태이며, 고순도(>90%)로 정제되었습니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 14-0242-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Immunohistochemistry (IHC) | Assay-Dependent | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 4 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | 53 publications |
| Functional Assay | Assay-Dependent | - |
| T-Cell Activation (TCA) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/69 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467170 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Available Formats
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 711 / 786
- eFluor 450
- FITC
- Functional Grade (Product #16-0242-85)
- NovaFluor Blue 510 / 610-30S
- NovaFluor Red 685 / 710
- NovaFluor Yellow 570 / 590 / 660
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436 / 600 / 645
- Request custom conjugation available
Product Specific Information
Description:
The M1/69 monoclonal antibody reacts with the mouse CD24 molecule (Heat Stable Antigen, HSA). This 35–50 kDa GPI-anchored molecule is expressed on erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage cells. CD24 is variably glycosylated, leading to heterogeneity in molecular mass across cell types. Expression of CD24 is used to determine stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.
Applications Reported:
The M1/69 antibody is suitable for flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. It has also been reported in in vitro functional assays. For functional assays, use the Functional Grade purified M1/69 (Product #16-0242-85).
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes and thymocytes. Recommended usage ≤0.125 µg per test (100 µL final volume). Optimal titration should be determined empirically (10⁵–10⁸ cells/test).
Target Information
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed during multiple stages of B-cell development, from pro-B cells to mature B cells. Plasma cell expression is low or negative. CD24 is also found in most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may regulate B-cell proliferation, maturation, and autoimmunity control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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