Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), eBioscience
인간 CD22를 인식하는 eBio4KB128 단클론 항체로, Flow Cytometry와 IHC에 적합합니다. 0.5 mg/mL 액상 형태이며 PBS 완충액에 0.09% sodium azide를 포함합니다. 높은 순도(>90%)로 제조되어 B세포 및 림프종 연구에 유용합니다.
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Thermo Fisher Scientific CD22 Monoclonal Antibody (eBio4KB128 (4KB128)), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-dependent |
| Flow Cytometry (Flow) | 0.5 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio4KB128 (4KB128) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864846 |
| Purity | >90%, determined by SDS-PAGE |
| Aggregation | <10%, determined by HPLC |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugate Formats
- APC
- FITC
- NovaFluor Blue 690
- NovaFluor Red 755
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Custom conjugation available upon request
Product Specific Information
The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of CD22α and CD22β chains (130 and 140 kDa), produced by alternative splicing. It is highly expressed on mature B cells and B-cell lymphomas.
The extracellular region contains seven Ig-like domains capable of binding sialic acid moieties on epithelial, endothelial, B, and T cells. The intracellular region includes six tyrosine residues within ITIM and activation-like motifs, which are phosphorylated upon B-cell receptor engagement, enabling CD22 to regulate B-cell receptor signaling positively and negatively.
Applications Reported: Suitable for flow cytometric analysis and immunohistochemical staining.
Applications Tested: Verified by flow cytometric analysis of normal human peripheral blood cells at ≤0.5 µg/test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Titration recommended for optimal performance.
Target Information
CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed in cytoplasm and membrane of B-lymphocytes. It appears early in B-cell differentiation, similar to CD19, and is limited to late differentiation stages (mature B cells to plasma cells).
CD22 is useful in phenotyping mature leukemias and is strongly expressed in hairy cell leukemia. It binds preferentially to α2,6-linked sialic acid, with cis interactions potentially masking the binding site. Upon ligand-induced tyrosine phosphorylation, CD22 regulates B-cell receptor signaling through both activation (via Src kinases) and inhibition (via SH2 domain phosphatases).
CD22 is positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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