Thermo Fisher Scientific Desmoglein 3 Monoclonal Antibody (3G133)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

20-50 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

5-10 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3G133

Immunogen

Recombinant fragment (Human Desmoglein 3 extracellular domain).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2093599

Product Specific Information

It does not cross react with Desmoglein 1 or 2.

A431 cells can be used as a positive control for this antibody.

Target Information

Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominately expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 has been characterized as the auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.