Thermo Fisher Scientific IL-9 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Murine IL-9 항체로, Western blot과 ELISA에 최적화되어 있습니다. 고순도 재조합 IL-9 항원으로 면역하여 높은 특이성과 감도를 제공합니다. 동결건조 형태로 -20°C 보관하며, 연구용으로 사용됩니다.
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- 500-P59BT-xxxx (2개 옵션)
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Thermo Fisher Scientific IL-9 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | — |
| ELISA | 0.25–1.0 µg/mL | 2 publications |
| Immunostaining (IS) | — | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-9 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930645 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MQRCSTTWGI RDTNYLIENL KDDPPSKCSC SGNVTSCLCL SVPTDDCTTP CYREGLLQLT NATQKSRLLP VFHRVKRIVE VLKNITCPSF SCEKPCNQTM AGNTMSFLKS LLGTFQKTEM QRQKSRP
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-9. Anti-Murine IL-9-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Murine IL-9 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-9 (500-P59) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-9.
Western Blot:
To detect Murine IL-9 by Western Blot analysis, this antibody can be used at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine IL-9 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P59BT-1MG is provided as 2 × 500 µg.
Target Information
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells.
Mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein (~14 kDa). Native mouse IL-9 is heavily glycosylated.
TGF-β plays a key role in differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, driving Th9 cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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