Thermo Fisher Scientific BAFF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hBAFF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BAFF (500-P163G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hBAFF.

Western Blot:
To detect hBAFF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hBAFF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P163GBT-1MG is provided as 2 × 500 µg.

Target Information

BAFF is a membrane-bound cytokine expressed by B cell lineage cells. The soluble form binds to receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, important for proliferation and differentiation of B cells. It is also expressed by T lymphocytes and promotes T cell activation and survival. Isoforms representing alternatively spliced and glycosylated variants have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.