Thermo Fisher Scientific BAFF Polyclonal Antibody, Biotin, PeproTech
Human BAFF 단백질을 인식하는 Biotin 결합 Goat Polyclonal 항체. Western Blot과 ELISA에 적합하며, 고순도 항원 친화 크로마토그래피로 정제됨. 최소 0.2 ng 수준의 hBAFF 검출 가능. 연구용으로만 사용.
- 카탈로그번호
- 500-P163GBT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific BAFF Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human BAFF (BAFF (BLyS), UniProt ID: Q9Y275-1, NCBI Node ID: 9606, Antigen Range: 134–285, Antigen Length: 285) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929579 |
Product Specific Information
AA Sequence of recombinant protein:
AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL.
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hBAFF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BAFF (500-P163G) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hBAFF.
Western Blot:
To detect hBAFF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hBAFF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Package Information:
500-P163GBT-1MG is provided as 2 × 500 µg.
Target Information
BAFF is a membrane-bound cytokine expressed by B cell lineage cells. The soluble form binds to receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, important for proliferation and differentiation of B cells. It is also expressed by T lymphocytes and promotes T cell activation and survival. Isoforms representing alternatively spliced and glycosylated variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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