Thermo Fisher Scientific CD25 Monoclonal Antibody (eBio7D4 (7D4)), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (eBio7D4 (7D4)), Alexa Fluor 488, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.06 µg/test
Publications: View 2 publications

Product Specifications

Additional Formats:

• Biotin
• eFluor 660
• Request custom conjugation

Product Specific Information

The eBio7D4 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Ra).
CD25 is expressed by early progenitors of the T and B lineage, as well as by activated mature T and B lymphocytes.
By itself, CD25 binds IL-2 only with low affinity, but associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high-affinity IL-2 receptor.
CD25 plays a role in lymphocyte differentiation and activation/proliferation.

The epitopes recognized by eBio3C7, PC61.5, and eBio7D4 are different, allowing depletion with one antibody (typically PC61.5) followed by detection with another.
eBio7D4 has been reported to block IL-2 binding, but can be overcome by additional IL-2.

The monoclonal antibody PC61.5 (Product #16-0251) is recommended for in vivo studies.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes.
Use ≤ 0.06 µg per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.

Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD25 (IL2RA) encodes the interleukin-2 receptor α chain, part of a heterotrimeric complex with β and γ chains forming the high-affinity IL-2 receptor.
IL2RA plays a key role in T and B lymphocyte proliferation, differentiation, and survival.
IL-2 stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation.
Mutations in IL2RA are associated with interleukin-2 receptor α deficiency and immune dysregulation.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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