Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

Unconjugated, Alexa Fluor 532, 561, 647, 660, 700, APC, APC-eFluor 780, Biotin, Brilliant UV 563/661/737/805, Brilliant Violet 421/650/711/786, eFluor 450/506, FITC, Functional Grade, NovaFluor Blue series, Pacific Orange, PE, PE-Cyanine5/5.5/7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436/600/645/702/780, Custom conjugation available.

Product Specific Information

Description:
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. CD25 binds IL-2 with low affinity alone, but forms a high-affinity receptor when associated with CD122 (IL-2Rβ) and CD132 (common γ chain). The PC61.5 antibody inhibits IL-2 binding to both high- and low-affinity receptors, playing a role in lymphocyte differentiation and proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤0.06 µg/test). A test corresponds to a 100 µL staining volume. Optimize antibody concentration empirically (10⁵–10⁸ cells/test).
Excitation/Emission: 488 nm / 519 nm (Blue Laser)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD25 (IL2 receptor alpha chain, IL2RA) is part of a heterotrimeric receptor complex (IL2RA, IL2RB, IL2RG) responsible for IL-2 signaling in T and B lymphocyte proliferation. IL2RA homodimers form low-affinity receptors, IL2RB homodimers form medium-affinity receptors, and the trimeric complex forms a high-affinity receptor. IL2 is primarily produced by mature T cells and functions as a growth and differentiation factor. Disruption of IL2RA in mice results in immune dysregulation similar to ulcerative colitis, highlighting its importance in immune homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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