Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 488, eBioscience
마우스 CD25(IL-2Rα) 인식 단클론 항체로, Alexa Fluor 488 결합 형식. Flow cytometry에 최적화되어 있으며 0.06 µg/test로 사용 가능. T 및 B 세포 활성화 연구에 적합하며, 4°C 암소 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | - | 1 |
| Flow Cytometry (Flow) | 0.06 µg/test | 99 |
| Neutralization (Neu) | - | 2 |
| Functional Assay | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_763475 |
Available Formats
Unconjugated, Alexa Fluor 532, 561, 647, 660, 700, APC, APC-eFluor 780, Biotin, Brilliant UV 563/661/737/805, Brilliant Violet 421/650/711/786, eFluor 450/506, FITC, Functional Grade, NovaFluor Blue series, Pacific Orange, PE, PE-Cyanine5/5.5/7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436/600/645/702/780, Custom conjugation available.
Product Specific Information
Description:
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. CD25 binds IL-2 with low affinity alone, but forms a high-affinity receptor when associated with CD122 (IL-2Rβ) and CD132 (common γ chain). The PC61.5 antibody inhibits IL-2 binding to both high- and low-affinity receptors, playing a role in lymphocyte differentiation and proliferation.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤0.06 µg/test). A test corresponds to a 100 µL staining volume. Optimize antibody concentration empirically (10⁵–10⁸ cells/test).
Excitation/Emission: 488 nm / 519 nm (Blue Laser)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD25 (IL2 receptor alpha chain, IL2RA) is part of a heterotrimeric receptor complex (IL2RA, IL2RB, IL2RG) responsible for IL-2 signaling in T and B lymphocyte proliferation. IL2RA homodimers form low-affinity receptors, IL2RB homodimers form medium-affinity receptors, and the trimeric complex forms a high-affinity receptor. IL2 is primarily produced by mature T cells and functions as a growth and differentiation factor. Disruption of IL2RA in mice results in immune dysregulation similar to ulcerative colitis, highlighting its importance in immune homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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