Thermo Fisher Scientific MCP-1 Monoclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

Preparation:
Produced in BALB/c mice immunized with highly pure Recombinant Human MCP-1 (CCL2). Anti-Human MCP-1 (CCL2)-specific antibody was purified from cell culture supernatant by Protein A affinity chromatography.

Sandwich ELISA:
Assuming 100 µL/well, a concentration of 1.0–2.0 µg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-1 (CCL2) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-1 (CCL2) (500-P34BT) as the detection antibody at approximately 0.5–1.0 µg/mL.

Western Blot:
To detect Human MCP-1 (CCL2) by Western Blot, use at 0.20–0.40 µg/mL. Detection limit for recombinant protein is 1.0–2.0 ng/lane under reducing and 0.25–0.50 ng/lane under non-reducing conditions.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. The encoded protein, related to the CXC subfamily of cytokines, is characterized by two cysteines separated by one amino acid. It shows chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It is implicated in diseases with monocytic infiltrates such as psoriasis, rheumatoid arthritis, and atherosclerosis, and binds to receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.