Thermo Fisher Scientific MCP-1 Monoclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.20-0.40 µg/mL

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

ELISA (ELISA)

1.0-2.0 µg/mL

View 9 publications 9 publications

Neutralization (Neu)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Bovine, Human, Mouse

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human MCP-1 (CCL2) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL2 (MCP-1),uniProtId:P13500-1,ncbiNodeId:9606,antigenRange:24-99,antigenLength:99,antigenImageFileName:500-M71-500UG_CCL2_MCP-1_P13500-1_House_mouse.svg,antigenImageFileNamePDP:500-M71-500UG_CCL2_MCP-1_P13500-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_3074168

Product Specific Information

AA Sequence of recombinant protein: QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT.

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human MCP-1 (CCL2). Anti-Human MCP-1 (CCL2)-specific antibody was purified from cell culture supernatant by Protein A affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 1.0-2.0 µg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-1 (CCL2) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-1 (CCL2) (500-P34BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.

Western Blot: To detect Human MCP-1 (CCL2) by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-1 (CCL2) is 1.0-2.0 ng/lane under reducing conditions and 0.25-0.50 ng/lane under non-reducing conditions.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.