Thermo Fisher Scientific CCL5 (RANTES) Monoclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS

Preparation:
Produced in BALB/c mice immunized with highly pure Recombinant Human RANTES (CCL5).
Anti-Human RANTES (CCL5)-specific antibody was purified from ascites fluid by Protein A affinity chromatography.

Sandwich ELISA:
Assuming 100 µL/well, a concentration of 2.0–4.0 µg/mL of this antibody will detect at least 500 pg/mL of Recombinant Human RANTES (CCL5) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human RANTES (CCL5) (500-P36BT) as the detection antibody at approximately 0.5–1.0 µg/mL.

Western Blot:
To detect Human RANTES (CCL5) by Western Blot, use at 0.5–1.0 µg/mL.
Detection limit for Recombinant Human RANTES (CCL5):

• 0.25–2.0 ng/lane (reducing conditions)
• 0.25–0.5 ng/lane (non-reducing conditions)

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are secreted proteins involved in immunoregulatory and inflammatory processes. CCL5, a member of the CC subfamily, acts as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It induces histamine release from basophils and activates eosinophils.
CCL5 is one of the major HIV-suppressive factors produced by CD8+ cells and functions as a natural ligand for CCR5, suppressing replication of R5 strains of HIV-1.
Alternative splicing produces multiple transcript variants encoding different isoforms.
Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.