Thermo Fisher Scientific MCP-3 Monoclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

-

ELISA (ELISA)

2.0-4.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929126

Product Specific Information

AA Sequence of recombinant protein: QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL.

Preparation: Produced in C3H/HeJ mice immunized with highly pure Recombinant Human MCP-3 (CCL7). Anti-Human MCP-3 (CCL7)-specific antibody was purified from ascites fluid by Protein A affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect Recombinant Human MCP-3 (CCL7) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-3 (CCL7) (500-P37BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.

Western Blot: To detect Human MCP-3 (CCL7) by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-3 (CCL7) is 2.0-4.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Target Information

This is a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.