Thermo Fisher Scientific IL-10 Monoclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

Preparation:
Produced in BALB/c mice immunized with highly pure Recombinant Human IL-10. Anti-Human IL-10-specific antibody was purified from tissue culture supernatant by Protein A affinity chromatography.

Sandwich ELISA:
Assuming 100 µL/well, a concentration of 8.0–10.0 µg/mL of this antibody will detect at least 2000 pg/mL of Recombinant Human IL-10 when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human IL-10 (500-P20BT) as the detection antibody at a concentration of approximately 0.05–0.10 µg/mL.

Western Blot:
To detect Human IL-10 by Western Blot analysis, this antibody can be used at a concentration of 0.25–0.50 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IL-10 is 4.0–8.0 ng/lane under reducing or non-reducing conditions.

Target Information

IL-10 encodes a cytokine primarily produced by monocytes, with some production by lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. IL-10 enhances B cell survival, proliferation, and antibody production, blocks NF-κB activity, and participates in JAK-STAT signaling regulation. Knockout studies in mice show IL-10 is essential for intestinal immune regulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis. Related diseases include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.