Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 737
• Brilliant Violet 421, 480, 650
• eFluor 450, 506, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600, 702, 780
• Request custom conjugation

Product Specific Information

Description:
The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits (gamma, delta, epsilon) are essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Binding of 17A2 to CD3 initiates intracellular signaling leading to activation and proliferation.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested on mouse splenocytes
• Recommended ≤ 1.0 µg/test (in 100 µL final volume)
• Optimize antibody titration for best performance

Fluorochrome Information:
Brilliant Ultra Violet™ 805 (BUV805) emits at 797 nm and is used with ultraviolet (355 nm) laser-equipped cytometers. Ensure your instrument supports this fluorochrome.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401) or
• Brilliant Stain Buffer (Product # 00-4409-75)
  to minimize non-specific interactions.

Light Sensitivity:
Protect from light; dye is sensitive to photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222)
• 1-step Fix/Lyse Solution (Product # 00-5333)
  Compatible with short-term methanol fixation (≤1 hour).

Fluorochrome Properties:

• Excitation: 355 nm
• Emission: 797 nm
• Laser: Ultraviolet

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, all part of the immunoglobulin superfamily. CD3 is expressed on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. It plays roles in TCR-induced growth arrest, survival, and proliferation. Defects in CD3 genes are linked to CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures.