Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: -

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 661, 737
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue/Red/Violet/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. CD3e, along with gamma and delta subunits, is essential for proper TCR assembly and surface expression. CD3 is expressed by thymocytes and mature T cells. Crosslinking via immobilized UCHT1 induces intracellular activation and proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test.

Brilliant Ultra Violet™ 805 (BUV805):

• Tandem dye emitting at 797 nm
• Excitation: 355 nm (UV laser)
• For cytometers equipped with ultraviolet laser

Buffer Recommendations:
When using polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333) for up to 3 days in the dark at 4°C. Compatible with short-term methanol fixation (<1 hour).

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It comprises CD3 gamma, delta, epsilon, and zeta subunits, encoded by genes on chromosome 11. CD3 is found on 68–82% of peripheral T lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 genes are linked to CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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