Thermo Fisher Scientific HIF1A Polyclonal Antibody, DyLight 550
HIF1A 단백질을 인식하는 DyLight 550 형광 표지 폴리클로날 항체로, 저산소 상태에서 HIF-1α 발현 분석에 적합. WB, IHC, ICC, Flow 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 안정성 제공.
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Thermo Fisher Scientific HIF1A Polyclonal Antibody, DyLight 550
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–10 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Dog, Fish, Goat, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein including residues 530–825 of mouse HIF-1 alpha protein |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.84 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152127 |
Product Specific Information
This antibody detects upregulation of HIF-1 alpha in hypoxic samples.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer composed of alpha and beta subunits, both belonging to the PAS family of transcription factors.
Under hypoxic conditions, HIF-1 activates transcription of over 40 genes involved in oxygen delivery and metabolic adaptation, including erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. It interacts with p53 and participates in MDM2-mediated ubiquitination under hypoxic conditions.
In humans, HIF1A is located on chromosome 14q. The C-terminal region binds to p300/CBP, forming complexes that induce hypoxia-responsive genes such as VEGF.
Hypoxia plays a key role in diseases such as ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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