Thermo Fisher Scientific HIF1A Polyclonal Antibody, DyLight 488
HIF1A 단백질을 인식하는 DyLight 488 형광 표지 폴리클로날 항체. 다양한 종에서 반응하며, WB, IHC, ICC, Flow 등 다중 응용 가능. 저산소 조건에서 HIF-1α 발현 검출에 적합. 항원 친화 크로마토그래피로 정제된 고순도 제품.
- 카탈로그번호
- PA522778
- 판매단위
- pk
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Thermo Fisher Scientific HIF1A Polyclonal Antibody, DyLight 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–10 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Dog, Fish, Goat, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein including residues 530–825 of the mouse HIF-1 alpha protein |
| Conjugate | DyLight™ 488 |
| Excitation / Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.81 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154069 |
Product Specific Information
This antibody detects upregulation of HIF-1 alpha in hypoxic samples.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer consisting of alpha and beta subunits, both members of the PAS family of transcription factors. It regulates the adaptive response to hypoxia by activating transcription of over 40 genes involved in oxygen delivery and metabolic adaptation, such as erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha plays a role in hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. Under hypoxic conditions, p53 interacts with HIF1-alpha, promoting MDM2-mediated ubiquitination and degradation. Induction of NOX4 by HIF1-alpha helps maintain ROS levels and hypoxia-induced proliferation. In humans, HIF1A is located on chromosome 14q and its C-terminal binds p300. The p300/CBP-HIF complex induces hypoxia-responsive genes including VEGF. Hypoxia contributes to diseases such as ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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