Thermo Fisher Scientific HIF1A Polyclonal Antibody, HRP
HIF1A 단백질을 인식하는 HRP 결합 폴리클로날 항체로, 저산소 조건에서 HIF-1α 발현 증가를 검출 가능. Western blot 및 IHC(F/P) 실험에 적합하며, 다양한 종에서 반응. 항원 친화 크로마토그래피로 정제된 고품질 연구용 시약.
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Thermo Fisher Scientific HIF1A Polyclonal Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Fish, Goat, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein including residues 530–825 of mouse HIF-1α protein |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 0.73 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_11155184 |
Product Specific Information
This antibody detects upregulation of HIF-1α in hypoxic samples.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer consisting of alpha and beta subunits, both belonging to the basic-helix-loop-helix PAS family of transcription factors.
HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia, activating transcription of over 40 genes including erythropoietin, glucose transporters, glycolytic enzymes, and VEGF.
HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. It interacts with p53 and p300/CBP complexes, playing a key role in hypoxia-responsive gene induction.
Located on chromosome 14q in humans, HIF1A is involved in pathophysiological processes such as ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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