Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), Biotin
Human Nestin 단백질을 인식하는 mouse monoclonal antibody로 Biotin 결합형 제품. 다양한 immunoassay(WB, IHC, ICC)에 적합하며 rodent 및 rabbit과 교차 반응하지 않음. 신경줄기세포 및 종양혈관세포 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Immunogen | A fragment of the human nestin protein corresponding to residues 1464–1614 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.02 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153888 |
Additional Formats
- DyLight 488 (MA5-16104)
- DyLight 550 (MA5-16106)
- DyLight 650 (MA5-16103)
- HRP (MA5-16105)
- Request custom conjugation
Product Specific Information
This antibody does not cross-react with rodent or rabbit.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also a superior angiogenic marker for evaluating neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin help characterize progenitor cells differentiating into distinct lineages, enhance therapeutic potential of human neural stem/progenitor cells in CNS disease or injury, and identify neuroepithelial tumor cells. Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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